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. 2023 Nov 22;624(7992):682–688. doi: 10.1038/s41586-023-06746-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 3 — a. Local resolution and particle distribution of the pre-1F overall and the locally refined pre-1F (left) and pre-1F (right) maps. b. Local resolution and particle distribution of the pre-2F overall and the locally refined pre-2F (left) and pre-2F (right) maps. c. 3DFSC analysis of the pre-1F overall, the focused refined pre-1F (left) and the focused refined pre-1F (right) reconstructions. d. 3DFSC analysis of the pre-2F overall, the focused refined pre-2F (left) and the focused refined pre-2F (right) reconstructions. e. FSC curves with the gold standard threshold of 0.143 for the three pre-1F RNP maps. f. Map to model FSC curve for the pre-1F model refined against the pre-1F overall map. g. FSC curves with the gold standard threshold of 0.143 for the three pre-2F RNP maps. h. Map to model FSC curve for the pre-2F model refined against the pre-2F overall map.