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. 2023 Oct 23;4(12):1675–1692. doi: 10.1038/s43018-023-00656-2

Fig. 3. Alterations in frequencies and distribution of Fc receptor expression on immune cell subsets in the bone marrow microenvironment of patients with AML.

Fig. 3

a, Schematic summary of FcγR expression across normal immune cell subsets. ITAM, immunoreceptor tyrosine-based activating motif; ITIM, immunoreceptor tyrosine-based inhibitory motif. b, Expression of activating receptor CD32A on monocyte populations in AML bone marrow (orange, n = 44 patients) compared to bone marrow from unaffected donors (blue, left, n = 6 donors) and representative flow cytometry histogram of CD32A expression on AML nonclassical monocytes (orange) compared to controls from unaffected donors (blue, right); P values are from Welch’s unpaired t-test. *P = 0.0182 (classical monocytes), *P = 0.0193 (immature monocytes), ****P < 0.0001 (left). c, Expression of activating receptor CD16 on CD56dim NK cells in AML bone marrow (n = 44 patients) compared to bone marrow from unaffected donors (left, n = 6 donors) and a representative flow cytometry histogram (right); P values are from Welch’s unpaired t-test. ***P = 0.0002 (left). d, Inhibitory receptor CD32B on B cells and monocytes in AML bone marrow (n = 44 patients) compared to bone marrow from unaffected donors (n = 6 donors) (top) and representative flow cytometry histograms (bottom); P values are from Welch’s unpaired t-test. **P = 0.0048 (classic memory B cells) and 0.0094 (non-naive B cells) (top). NS, not significant. e, Representative UMAP overlay generated from the 36-color spectral flow cytometry panel comparing normal bone marrow (blue) and AML bone marrow (orange, left) and individual representative UMAPs depicting classical monocyte and cDC2 cell populations (each demarcated with a red outline) in an unaffected control donor (top) compared to a patient with AML (bone marrow) in whom these populations are absent (bottom). f, Quantification of classical monocytes and cDC2 cells in AML bone marrow (n = 49) compared to bone marrow from control donors (n = 7 donors); P values are from Welch’s unpaired t-test. **P = 0.0028 (classical monocytes), **P = 0.0014 (cDC2) and ****P < 0.0001 (pDC). Data are mean ± s.e.m.

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