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Northern blot analysis of rpoS (A) and hrpNEcc (B) mRNA in E. carotovora subsp. carotovora AC5006 (lane 1) and its RpoS− derivative, AC5061 (lane 2). Total RNA was extracted from bacteria grown in minimal salts medium supplemented with sucrose (0.5% [wt/vol]) to a Klett value of 200. Each lane contained 10 μg of total RNA.
