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. 2023 Oct 16;30(12):2462–2476. doi: 10.1038/s41418-023-01223-z

Fig. 7. Phosphorylation of NSUN5 by CDK13 enhances lipid deposition.

Fig. 7

A PC3 and C4-2 cells were transfected with oeCDK13 and then treated with 1NM-PP1 (10 μM). Western blot examined NSUN5, p-NSUN5 and ACC1 protein expression. B Western blot analysis detected NSUN5 and p-NSUN5 expression in BPH and PCa tissues. C C4-2 cells were transfected with the vector encoding NSUN5 (WT) or different phosphorylation-deficient NSUN5 mutants, and then Western blotting examined NSUN5, p-NSUN5 and ACC1 protein level. D PC3 and C4-2 cells were co-transfected with or without oeNSUN5, oeNSUN5-S327A mutant or oeCDK13, and then Western blotting detected p-NSUN5 and ACC1 expression. E C4-2 cells were transfected with oeNSUN5 or oeNSUN5-S327A, and then CoIP-Western blotting detected the interaction between CDK13 and NSUN5. F C4-2 cells were co-transfected with the indicated constructs and then lipid accumulation was detected by ORO straining. Scale bar = 20 μm. G Quantitative analysis of ORO staining in (F). H PC3 and C4-2 cells were treated as in (F), and then TG and cholesterol contents were measured as quantitative indicators of lipid deposition. All data are expressed as the mean ± SEM of 3 independent experiments. *P < 0.05, **P < 0.01 vs. their corresponding controls.