Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Dec 20;14:8468. doi: 10.1038/s41467-023-44018-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — a An image of EGFP-xTalin1 SiMS acquired with 100-ms exposure time and unattenuated 75-W xenon illumination. b Representative displacement plots of the central position of EGFP-xTalin1 (N-tag, left) and xTalin1-EGFP (C-tag, right) in the series of fast-tracking images. The central position was determined by the Gaussian-Fit model of Speckle TrackerJ software. The EGFP-xTalin1 SiMS and the xTalin1-EGFP SiMS switch the motion from stationary to flowing. Insets show trajectories of the same xTalin1 SiMS. c Box-and-whisker plots of the distance (Δx) in the flow direction between the position at the stationary state and the first position where the flow is detected. Box-and-whisker plot shows the median (the line in the box), the 25th and 75th percentiles (box), and the minimum and maximum values (whiskers). Data represents Δx of n = 31 EGFP-xTalin1 SiMS (blue dots) from seven independent experiments and those of n = 29 xTalin1-EGFP SiMS (red dots) from 6 independent experiments. The p value (0.00190) was determined using a two-tailed t-test. In our estimation, xTalin1 SiMS that jumps 40 nm or more can be measured with more than 75% probability (Fig. S3). d The Δx distribution of EGFP-xTalin1 SiMS shown in (c). e A representative trajectory of the central position of xTalin1-EGFP SiMS that exhibits back-and-forth motion. The dots indicate the central position of the speckle for each frame and are connected by lines (85 frames). The dots and lines are color-coded by the time after the start of tracking, as indicated. Inset shows the trajectory of a speckle flowing nearby, shown in the same color-coding. f Displacement plot of the central position of xTalin1-EGFP SiMS shown in (e). g Summary of switching kinetics of xTalin1 engaging and disengaging via an elastic transient clutch in Talin. k_a represents the rate constant for the transition of stationary xTalin1 SiMS to a clutch state. k_b represents the rate constant for the transition of xTalin1 SiMS from clutching to a stationary state. k_c represents the rate constant for transition of clutching xTalin1 SiMS to a flowing state. k_d represents the rate constant for the transition of flowing xTalin1 SiMS to a stationary state. Source data are provided as a Source Data file.