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. 2023 Dec 20;14:8484. doi: 10.1038/s41467-023-44138-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Confocal images showing Lgr5 ISH (red) and DAPI (grey) staining in control (0%) and 2.5% DSS-treated murine colonic crypts. White arrows indicate Lgr5^+CBC cells. b Bar graphs showing the mean (±SEM) of Lgr5^+CBC cells per crypt between control and DSS-treated mice (n = 40 crypts counted from 3 animals/group). P-values from t-tests are indicated. c Bar graphs showing the mean (±SEM) TUNEL⁺ cells per 100 crypts (apoptotic index) at the crypt base (left) and above crypt base (right) in mice (n = 3 animals/group). P-values from the two-tailed Wilcoxon rank-sum test are given. d Confocal images showing Lgr5 ISH (red) and DAPI (grey) in NMR colonic and duodenal crypts. White arrows indicate Lgr5^+CBC cells. e Scatter graph showing mean (±SEM) Lgr5 mRNA expression levels at specific cell positions along the crypt axis in control and DSS-treated NMRs (n = 50 crypts counted from 3 animals/group). f The mean (±SEM) TUNEL⁺ cells per 100 crypts at the crypt base (left) and above crypt base (right) in NMRs (n = 4 animals analysed in 0% and 3 animals in 2.5% DSS-treated group). P-values generated from the two-tailed Wilcoxon rank-sum test are indicated. g, h Bar graphs showing mean percentage (±SEM) of Ki67-positive cells in Lgr5^+CBC cells per crypt in (g), mouse colon and duodenum (n = 40 crypts from 3 animals/group) and in (h), NMR duodenum (n = 30 crypts from 3 animals/group). P-values from t-tests are indicated. i, j Bar graph showing mean percentage (±SEM) of Ki67⁺ cells in all epithelial cells in the intestinal crypts of control and treated (i) mice (n = 40 crypts from 3 animals/group) and (j) NMRs (n = 30 crypts from 3 animals/group). P-values from t-tests are indicated. k Schema showing the recovery period in NMRs after 2.5% DSS treatment (green) and times of tissue collection (red). l Line graph showing the mean number (±SEM) of Lgr5^+CBC cells in the duodenum (black) and colon (grey) of NMRs after DSS withdrawal (n = 20 crypts from 1 animal at each time-point). Where indicated, Student’s t-test using two-tailed, unpaired and unequal variance was employed. Scale bars are indicated on the images (25 µm).