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. 2023 Dec 20;14:8461. doi: 10.1038/s41467-023-44312-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a, b Bar graph (a) and violin plots (b) showing the doublet ratios, nUMI, nGene, and log10(GenesPerUMI) among the EC subclusters. c, d Flow cytometry analysis illustrating the gating strategy (c) and quantification (d) of Tomato⁺ cells, Tomato⁺CD31⁺ cells, Tomato⁺CD31⁺CDH5⁺TIE2⁺ cells, and the doublet ratios of these cell populations in the live, Lin⁻CD45⁻ bone marrow cells of Prrx1-Cre;R26T (n = 3 biologically independent animals) and Lepr-Cre;R26T (n = 3 biologically independent animals) mice. Data represent the mean ± S.E.M. Statistical significance was determined by two-tailed Wilcox rank-sum test (b), two-tailed unpaired Student’s t-test or one-way ANOVA with Dunnett’s test (d). Source data are provided as a Source Data file.