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. 2023 Dec 20;14:8461. doi: 10.1038/s41467-023-44312-w

Fig. 5. Different EC lineage tracing models label distinct stromal cell subpopulations in vivo.

Fig. 5

a–e Representative immunostaining images showing the differential labeling of endosteal bone-lining cells (a), RUNX2+ endosteal osteolineage cells (b), osteocytes in the cortical bone (c), trabecular bone-lining cells (d), and metaphyseal stromal cells (e) (arrows) by Cdh5-tetO-Cre;R26T (n = 3 biologically independent animals) and Tek-CreERT2-R26T (n = 3 biologically independent animals) models. f–j Quantification of Tomato+ endosteal bone-lining cells (f), RUNX2+ endosteal osteolineage cells (g), osteocytes in the cortical bone (i), trabecular bone-lining cells (j), and metaphyseal stromal cells (i) traced in the Cdh5-tetO-Cre;R26T and Tek-CreERT2-R26T models. Scale bars: 10 µm. Data represent the mean ± S.E.M. Statistical significance was determined by two-tailed unpaired Student’s t-test (f–h adjusted for unequal variances with Welch’s test). Source data are provided as a Source Data file.