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. 2023 Dec 20;14:8491. doi: 10.1038/s41467-023-44176-0

Fig. 2. The transcriptional signatures IRU CAR-T cells in the presence of CsA in vitro.

Fig. 2

a–d Bulk RNA Sequencing analysis for four groups (WT/ IRU CAR-T cells (+/- CsA), n=3 technical replicates, 1 donor upon stimulation with 10-fold irradiated (50 Gy) Nalm6-FFluc-GFP). a PCA depicted the clustering of the transcriptome profile of WT/IRU CAR-T cells (+/- CsA). b Volcano plots showed the DEGs in WT/IRU CAR-T (+CsA) groups. c KEGG analysis showed the up- and downregulated gene sets in WT/IRU CAR-T (+CsA) groups. d Heatmap demonstrated the DEGs in cytokine and chemokine among the WT/IRU CAR-T cells with/without CsA. e, f qPCR validation of representative genes in bulk RNA-seq (n = 3 technical replicates, 1 donor. Data from the other two donors can be found in the source data file). All data are means ± s.d. P values were determined by two-tailed Unpaired t test (e, f). WT CAR-T (-CsA) as blue circle, WT CAR-T (+CsA) as blue square, IRU CAR-T (-CsA) as red circle and IRU CAR-T (+CsA) as red square.