TABLE 1.
Initial specific activities of resorcinol-hydroxylating and HHQ-dehydrogenating enzymes after fractionation of the cell extract by ultracentrifugation (100,000 × g, 1 h)a
| Prepn | Protein (mg ml−1) | Activity
|
|||
|---|---|---|---|---|---|
| Resorcinol hydroxylating
|
HHQ dehydrogenating
|
||||
| Sp act (mU mg−1) | Vol act (mU ml−1) | Sp act (mU mg−1) | Vol act (mU ml−1) | ||
| Cell extract | 16.6 | 23.0 | 375.1 (100%) | 45.0 | 742.6 (100%) |
| Cytosolic fraction | 12.2 | <0.1 | <0.1 | 10.1 | 122.0 (16%) |
| Membrane fraction | 3.2 | 65.2 (2.8) | 206.2 (55%) | 175.1 (3.9) | 554.2 (75%) |
| Membrane fraction of benzoate-grown cells | 1.2 | 9.3 | 20.8 | ||
a
HHQ dehydrogenation was measured at pH 8.0. Protein concentrations of membrane fractions of resorcinol- and benzoate-grown cells applied in the assays were equal. Percentages in parentheses indicate yields; other values in parentheses represent enrichment factors.