TABLE 1.
PCR primers used in this study
| Primer | Sequence (5′→3′) |
|---|---|
| LmsigB-1 | AC(A/G) TGC ATT TGA (C/G)(A/T)T A(A/T)A CCG A |
| LmsigB-2 | G(C/T)G T(A/T)C ATG T(G/T)C CGA GAC G |
| LmsigB-3 | TTT GCG GCG AGC TTT GTA AT |
| LmsigB-4 | CAT CGG TGT CAC GGA AGA AGA A |
| LmsigB-10 | CCC GTT TCT TTT TGA CTG C |
| LmsigB-12 | CAT TTA TTG AAA ATC GCA GTC |
| LmsigB-15 | AAT ATA TTA ATG AAA AGC AGG TGG AG |
| LmsigB-16 | ATA AAT TAT TTG ATT CAA CTG CCT T |
| LmsigB-17 | ACT TGA TAT ACT TCA AGT GCT |
| LmsigB-18 | GGG TAT AGT ACT TCG AAT CG |
| LmsigB-19 | CGA ATA TTA CCC ATA CCA G |
| LmsigB-20 | TAT GAT TGG GAT GGA AG |
| LmrsbW-1 | AAG GG(A/T) GAC AG(C/T) TTT GA(C/T) T(A/T)T GA |
| LmrsbW-3 | GGC ATT CTC CTC CAC CTG |
| LmrsbW-4 | CTC AAC CTG ATA AAG AGG CG |
| SOE-Aa | GGA ATT CCA GGT GGA GGA GAA TGC |
| SOE-Bb | CAC ACG TTC AAA TCC ATC ATC TGC AGC AAA TGC TTC AAA G |
| SOE-C | GAT GAT GGA TTT GAA CGT GTG |
| SOE-Dc | GCT CTA GAA CAT CCC CGC AGT ATT G |
a
The EcoRI restriction site incorporated into this primer to facilitate cloning is underlined.
b
The overhang complementary to SOE-C is underlined.
c
The XbaI restriction site incorporated into this primer to facilitate cloning is underlined.