Table 1.

Genes and the primer sequences (with the Illumina overhand adapter sequences) used for the quadplex PCR amplicon sequencing assay.

Gene	Primer name	Primer sequences	Amplicon size	References
gltA	BhCS781.p (ap,s)	TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGGGGGACCAGCTCATGGTGG	338 bp	Norman et al., 1995
	BhCS1137.n (ap,s)	GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGAATGCAAAAAGAACAGTAAACA
groEL	groEL_Forward	TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGATAKCCACGATCAAACTGCAT	339 bp	This study
	groEL_Reverse	GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGTGTTGCGTGAAGTTGCTTCT
rpoB	prAPT0244	TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGGATGTGCATCCTACGCATTATGG	408 bp	Oksi et al., 2013
	prAPT0245	GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGAATGGTGCCTCAGCACGTATAAG
ssrA	ssrA_Forward	TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGGCTATGGTAATAAATGGACAATG	262 bp	This study
	ssrA_Reverse	GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGCTTCTGTTGCCAGGTG		Diaz et al., 2012