Table 3.
Discrimination of co-occurred Bartonella species using the quadruplex PCR amplicon sequencing.
| Bartonella henselae + Bartonella koehlerae | Bartonella henselae + Bartonella koehlerae + Bartonella grahamii | ||||
|---|---|---|---|---|---|
| Bartonella henselae | Bartonella koehlerae | Bartonella henselae | Bartonella koehlerae | Bartonella grahamii | |
| gltA | Yes | Yes | Yes | x | Yes |
| groEL | Yes | Yes | Yes | Yes | x |
| rpoB | Yes | Yes | Yes | Yes | Yes |
| ssrA | Yes | Yes | Yes | Yes | Yes |
Three replicates of each sample were tested. Yes, identical sequences of the target for the Bartonella species were obtained for all three replicates. x, sequences of the target for the Bartonella species were obtained in ≤ 2 replicates.