Table 4.

Performance comparison of the quadruplex PCR amplicon sequencing on Bartonella quintana- and B. triborcorum-DNA derived from different sample types.

	Culture-derived Bartonella quintana DNA	Lice-derived Bartonella quintana DNA	Culture-derived Bartonella tribocorum DNA	Rat spleen-derived Bartonella tribocorum DNA
gltA	Yes	Yes	Yes	Yes
groEL	x	x	Yes	Yes
rpoB	Yes	Yes	Yes	x
ssrA	Yes	Yes	x	x

Three replicates of each sample were tested. Yes, identical sequences of the target for the Bartonella species were obtained for all three replicates. x, sequences of the target for the Bartonella species were obtained in ≤ 2 replicates.