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. 2023 Nov 25;5(1):vdad152. doi: 10.1093/noajnl/vdad152

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© The Author(s) 2023. Published by Oxford University Press, the Society for Neuro-Oncology and the European Association of Neuro-Oncology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — Digital reconstructions of patient-derived glioblastoma organoid (PGO) slides after multiplex immunohistochemistry, cells were classified based on marker expression: MES (mesenchymal-like), OPC (oligodendrocyte-progenitor like), AC (astrocyte like), and NPC (neural progenitor cell like) or SOX2 (no specific classification). (A) PGO.007. (B) PGO.009. (C) PGO.027. (D) PGO.030. (E) PGO.031. (F) PGO.033. (G) Quantification of different cell populations in GBM organoids (blank bars) and their corresponding parental tumors (dashed bars). (H) Digital reconstructions of epidermal growth factor receptor (EGFR) expression heterogeneity within PGOs, representative examples are shown from a PGO with high EGFR and low EGFR expression.