TABLE 4.
Transformation mapping of suppressor strains
| Recipient | Absence or presence of pinpoint colonies with indicated donor DNAa
|
Geneb | Allelec | ||||||
|---|---|---|---|---|---|---|---|---|---|
| pRR7d | pRS11d | pRT2d | pRU13d | pRV6d | pDT11e | pDU3e | |||
| XS1 | − | − | − | − | + | + | − | rsbV | rsbV74GD |
| XS2 | − | − | − | + | + | − | + | rsbU | rsbU44PR |
| XS4 | − | − | − | − | + | + | − | rsbV | rsbV85LP |
| XS5 | − | − | − | + | + | − | + | rsbU | rsbU80Yterm |
| XS6 | − | − | + | + | + | 0 | − | rsbT | rsbT107VG |
| XS15 | − | − | − | + | + | + | + | rsbU | rsbU194VA |
| XS16 | − | − | + | + | + | 0 | − | rsbT | rsbT15IS |
| XS33 | − | − | + | + | + | 0 | − | rsbT | rsbT63Qterm |
| XS35 | − | − | + | + | + | 0 | − | rstT | ΔrsbST |
| XS38 | − | − | − | + | + | − | + | rsbU | rsbU228GR |
a
−, absence; +, presence; 0, no transformant colonies.
b
Predicted site of mutation.
c
Alterations detected by DNA sequencing. Numbers following the gene designations indicate the amino acid residues at which the changes occurred, followed by the original and new amino acids at these sites (e.g., rsbV74GD is a glycine-to-aspartate change at position 74 of rsbV). Changes which created nonsense codons are designated “term.” ΔrsbST is an rsbS-to-rsbT deletion.
d
Linearized plasmid.
e
Intact replicating plasmid for overexpression of rsbT (pDT11) or rsbU (pDU3).