Table 2. Primer Used for the Quantitative Reverse Transcription Polymerase Chain Reactiona.
| primer
sequence |
|||
|---|---|---|---|
| gene name | forward | reverse | references |
| TNF-α | 5′-GTGGTCAGGTTGCCTCTGTCTC-3′ | 5′-TGGCTCTGTGAGGAAGGCTGTG-3′ | (44) |
| IL-1β | 5′-TTTCCTCCTTGCCTCTGATGGG-3′ | 5′-CCACACGTTGACAGCTAGGTTC-3′ | (44) |
| IL-6 | 5′-CTTGGGACTGATGCTGGTGACA-3′ | 5′-GCCTCCGACTTGTGAAGTGGTA-3′ | (44) |
| SOD-1 | 5′-TGCGTGCTGAAGGGCGAC-3′ | 5′-GTCCTGACAACAACCTGGTTC-3′ | (45) |
| SOD-2 | 5′-GGAGCAAGGTCGCTTACAGA-3′ | 5′-GTGCTCCCACACGTCAATC-3′ | (45) |
| iNOS | 5′-GGACGAGACGGATAGGCAGAGA-3′ | 5′-TCTTCAAGCACCTCCAGGAACG-3′ | (44) |
| β-actin | 5′-CGTGAAAAGATGACCCAGATCA-3′ | 5′-ACAGCCTGGATGGCTACGTA-3′ | (16) |
a
TNF-α, tumor necrosis factor – α; IL-1β, interleukin-1β; IL-6; SOD-1, superoxide dismutase-1; SOD-2, superoxide dismutase-2; iNOS, inducible nitric oxide synthase.