Fig. 5.

DH delivery, kinetics, and trafficking of CoQ10@TNPs. A The schematic describes two different ways to deliver CoQ10@TNPs to DH. B, C The content of CoQ10@TNPs in the DH of the two groups was determined by organ in vivo imaging. D–H The content of CoQ10@TNPs in the lung, liver, spleen, and kidney of the two groups was determined by organ in vivo imaging. I, J The content of CoQ10@NPs and CoQ10@TNPs in DH was determined by organ in vivo imaging. K The content of CoQ10 in DH tissues was evaluated by HPLC. L The content of CoQ10 in the mitochondria of DH was evaluated by HPLC. M Schematic of DH perfused with CoQ10@TNPs. N DH underwent flushing in situ with CoQ10@TNPs via a tiny tube (5 × 1 mm) in the ascending aorta and IVC. O The DH was implanted into the neck of the recipient. P, Q The content of CoQ10@TNPs in DH was determined by organ in vivo imaging before transplantation. R, S The content of CoQ10@TNPs in DH was determined by organ in vivo imaging 24 h after transplantation. Data are represented as the mean ± SD (n = 4). ns: no significance; *p < 0.05; **p < 0.01; ***p < 0.001