Fig. 1.

Detection of csi-miR-96-5p in CS-infected patients with ICC. a Design and validation of cross-species primers for csi-miR-96-5p and hsa-miR-96-5p. b, c Verifying the specificity of csi-miR-96-5p and hsa-miR-96-5p primers. Total RNA samples for CS worms and HuCCT1 cells were 500 ng. The same qPCR conditions were used for the two groups. c Expression of csi-miR-96-5p and hsa-miR-96-5p in patients with ICC. The control group refers to disease-free tissues from patients who underwent hepatectomy because of trauma. Data are presented as the mean ± SD from three replicates, *P < 0.05, **P < 0.01. ICC, intrahepatic cholangiocarcinoma; CS, Clonorchis sinensis; NC, negative control without CS infection; AD, tumor-adjacent normal tissue; SD, standard deviation; qPCR, quantitative polymerase chain reaction