Fig. 2.

Prediction and validation of PTEN as a csi-miR-96-5p target gene. a The putative binding region of csi-miR-96-5p for PTEN. b The luciferase reporter gene method was used to determine the PTEN gene reporter activity. c, d csi-miR-96-5p mimics were successfully transfected into HuCCT1 cells. e–g PTEN mRNA and protein levels after transfection with csi-miR-96-5p mimics or NC mimics were detected using qPCR and WB, respectively. h Pearson correlation analysis of csi-miR-96-5p and PTEN levels in tissues from patients with CS-ICC. Data are presented as the mean ± SD from three replicates, *P < 0.05, **P < 0.01. ICC, intrahepatic cholangiocarcinoma; CS, Clonorchis sinensis; SD, standard deviation; ns, no statistical difference; qPCR, quantitative polymerase chain reaction