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. 2023 Dec 20;16:465. doi: 10.1186/s13071-023-06075-7

Fig. 4.

Fig. 4

Extraction and validation of csi-miR-96-5p in CsEVs. a Transmission electron microscopy analysis of CsEVs. b Nanoparticle tracking analysis of the sizes of CsEVs. c Western blot analysis of characteristic CsEV proteins (CD63 and TSG101). d CsEVs labeled with PKH-67 can be taken up by HuCCT1 cells. e qPCR analysis of the level of csi-miR-96-5p in cells treated with CsEVs. CsEVs, Clonorchis sinensis secreted extracellular vesicles; qPCR, quantitative polymerase chain reaction. *P < 0.05, **P < 0.01