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HPLC analysis of substrate conversion. Conversion from 5KG to d-gluconate by 5-keto-d-gluconate 5-reductase (A), from 5KG to l-idonate by l-idonate 5-dehydrogenase (B), and from 5KG to both l-idonate and d-gluconate by 5-keto-d-gluconate 5-reductase and l-idonate 5-dehydrogenase (C) are shown. Authentic l-idonate standard (D) is also shown.
