TABLE 2.
Strains and plasmids used in this study
| Strain or plasmid | Relevant genotype, phenotype, or characteristic | Source or reference |
|---|---|---|
| Strains | ||
| W1485 | K-12 wild type | CGSCa |
| DH5α | lacZΔM15 recA | BRLb |
| NP202 | Δ(gntR gntK gntU) | This study |
| NP250 | ΔidnR | This study |
| CB350 | ΔidnD recD | This study |
| TUG287 | F−gntK gntV his trp | 33 |
| DPB271 | recD | 24 |
| P90C | ara Δ(pro-lac) thi | 30 |
| BM129 | Δ(edd) | This study |
| M15 | recA+uvr+ F−mlt gal ara lac (pREP4) | Qiagen, Inc. |
| Plasmids | ||
| pCB95 | idnO | This study |
| pCB96 | idnD | This study |
| pCB98 | idnT | This study |
| pCU102 | idnT in pQE30 (idnT controlled expression) | This study |
| pNP204 | idnK idnD idnO idnT idnR′ | |
| pUC18 | bla lacZ′c | This study |
| pUC19 | bla lacZ′ | This study |
| pBR322 | Tcrbla | |
| pQE30 | Expression vector; His6 affinity tag | Qiagen, Inc. |
a
CGSC, E. coli Genetic Stock Collection.
b
BRL, Bethesda Research Laboratories.
c
Incomplete genes.