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Inhibition of gluconate transport (IdnT) by alternative substrates
| Competing sugara | Rate (nmol/min/mg) | % of wild-type rate |
|---|---|---|
| None (control) | 2.13 | 100 |
| d-Gluconic acid | 0.30 | 14 |
| l-Idonic acid | 0.42 | 14 |
| d-Glucose | 3.58 | 168 |
| 5-Keto-d-gluconic acid | 1.71 | 80 |
| d-Glyceric acid | 1.40 | 66 |
| l-Iduronic acid | 2.09 | 98 |
Cells preincubated in 2 mM competing sugar prior to uptake of 200 μM [14C]gluconate.
