TABLE 5.
Induction of IdnO and GntK activities in E. coli W1485 cellsa
| Carbohydrate (0.2%) | IdnO sp actb
|
IdnK sp act,b LB | |
|---|---|---|---|
| LB | M63c | ||
| None | 10.0 | NTd | <0.01 |
| d-Glucose | 16.0 | 6.2 | NT |
| d-Gluconate | 12.5 | 8.0 | 1.3 |
| 5KG | 825.0 | 135.0 | 5.7 |
| 5KG + d-glucose | NT | 78.0 | NT |
| l-Idonate | 158.0 | 99.0 | NT |
| l-Idonate + d-glucose | NT | 69.3 | NT |
Cells all harvested during log phase.
Expressed as nanomoles per minute per milligram of total cell protein.
M63 minimal medium.
NT, not tested.