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. 2023 Dec 19;22:15330338231218163. doi: 10.1177/15330338231218163

Figure 3.

Figure 3.

Knockdown of COLEC12 reduces cell proliferation, migration, and invasion in GC cells. (A) Western blot analysis was performed to detect COLEC12 expression in normal gastric epithelial cell line GES-1 and human GC cell lines (HGC-27, MKN-45, MGC-803, and AGS) (n  =  3). (B, C) Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot analysis demonstrated the knockdown of COLEC12 in HGC-27 and MKN-45 cells via transient transfection with siCOLEC12. (D) Cell proliferation assay after COLEC12 knockdown (n  =  3). (E) Colony formation assays after the COLEC12 knockdown (n  =  3). (F) Migration assay after COLEC12 knockdown (n  =  3). (G) Invasion assay after the COLEC12 knockdown (n  =  3). Representative images are shown at 200 × magnification; Scale bar = 100 µm. Error bars represent SD. Data represent 3 independent experiments. *P < .05, **P < .01, and ***P < .001. COLEC12, Collectin subfamily member 12; GC, gastric cancer.