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Physical map of the 5.7-kb insert of plasmid pCAH32. The location of the Ptac promoter and the glutathione S-transferase (GST) gene of the cloning vector pGEX-3X and the orientation of the insert are depicted. Restriction sites for the following endonucleases are noted: E, EcoRI; B, BamHI (open letter indicates cloning site); X, XmnI.
