Fig 3.

rAAV2.5 transduction of individual gene knockout HeLa cells. Eleven candidate genes (KIAA0319L, TM9SF2, ZNF320, KYAT3, NPHP3, WDR63, PDE4A, OR51M1, GPR174, CCDC92, and MSMO1) were selected based on the shRNA knockdown screening. A gRNA-expressing lentiviral vector was applied to HeLa cells, and single-cell cloning was carried out to generate a knockout cell line. (A) Western blotting. Western blots show the knockout efficiency at 3 days post-transduction. β-Actin was used as a loading control. (B) Luciferase activities in gene knockout HeLa cells. Gene knockout cells were transduced with rAAV2.5T at an MOI of 20,000 DRPs/cell. At 3 days post-transduction, the luciferase activities were measured. The red dashed line indicates 50% of the luciferase activity in non-target control HeLa cells. Data shown are means with an SD from three replicates.