TABLE 1.
Effects of temperature and H-NS mutation on LT expression
| Genotype | Strain (plasmid) | Temp (°C) | OD410a
|
|
|---|---|---|---|---|
| Mid-log phaseb | Late-log phaseb | |||
| hns+ | GM37(pLT) | 37 | 0.32 | 0.45 |
| hns+ | GM37(pLT) | 18 | 0.13 | 0.001 |
| hns | GM230(pLT) | 37 | 0.50 | 0.60 |
| hns | GM230(pLT) | 18 | 0.60 | 0.40 |
a
OD410s measure the amounts of LT protein determined by GM1-ELISA. See text for details.
b
Sample extracts were derived from the same cultures at different points in the growth curve. Replicate OD410s for each sample extract were obtained. The reported values are the averages of these determinations.