TABLE 1.
All strains and plasmids used in this study
| Name | Genotype | Description | Reference |
|---|---|---|---|
| Escherichia coli strains | |||
| DH5α | F- Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17 (rK-,mk+) phoA supE44 λ-thi- gyrA96 relA1 | Used for creating and maintaining plasmids | Bethesda Research Laboratories |
| S17-1 | recA pro hsdR RP4-2-Tc::Mu-Km::Tn7 | Used for mobilizing plasmids into N. aromaticivorans via conjugation | (49) |
| N. aromaticivorans strains | |||
| 12444Δ1879 | DSM 12444 ΔSaro_1879 | Parent strain; putative sacB has been deleted to allow genomic modifications using a sacB-containing plasmid | (3) |
| 12444ΔcrtB | DSM 12444 ΔSaro_1879 ΔSaro_1814 | Parent with deleted crtB | This work |
| 12444ΔcrtY | DSM 12444 ΔSaro_1879 ΔSaro_1817 | Parent with deleted crtY | This work |
| 12444ΔcrtG | DSM 12444 ΔSaro_1879 ΔSaro_0236 | Parent with deleted crtG | This work |
| 12444ΔcrtGZ | DSM 12444 ΔSaro_1879 ΔSaro_0236 ΔSaro_1168 | Parent with deleted crtG and crtZ | This work |
| 12444StaxiW | DSM 12444 ΔSaro_1879 ΔSaro_0236::crtW from S. taxi ATCC 55669 | Parent with deleted crtG, with the gene for the CrtW protein from Sphingomonas taxi ATCC 55669 (NCBI Accession WP_038660513.1) in the Saro_0236 genomic locus | This work |
| 12444SastaW | DSM 12444 ΔSaro_1879 ΔSaro_0236::crtW from S. astaxanthinifaciens | Parent with deleted crtG, with the gene for the CrtW protein from Sphingomonas astaxanthinifaciens (NCBI accession WP_211248127.1) in the Saro_0236 genomic locus | This work |
| 12444PDCΔdmtS | DSM 12444 ΔSaro_1879 ΔSaro_2819 ΔSaro_2864-5 ΔSaro_1872 | Parent with deleted ligI, desCD, and dmtS that accumulates PDC from aromatic monomers | (7) |
| PDCΔcrtG | DSM 12444 ΔSaro_1879 ΔSaro_2819 ΔSaro_2864-5 ΔSaro_1872 ΔSaro_0236 | 12444PDCΔdmtS with deleted crtG | This work |
| PDCΔcrtGZ | DSM 12444 ΔSaro_1879 ΔSaro_2819 ΔSaro_2864-5 ΔSaro_1872 ΔSaro_0236 ΔSaro_1168 | 12444PDCΔdmtS with deleted crtG and crtZ | This work |
| PDCSastaW | DSM 12444 ΔSaro_1879 ΔSaro_2819 ΔSaro_2864-5 ΔSaro_1872 ΔSaro_0236::crtW from S. axtaxanthinifaciens | 12444PDCΔdmtS with deleted crtG, with the gene for the CrtW protein from S. astaxanthinifaciens in the Saro_0236 genomic locus | This work |
| Plasmids | |||
| pK18msB-MCS1 | pK18mobsacB lacking the multiple cloning site, with a new XbaI site introduced | (3, 48) | |
| pK18msB/ΔSaro1814 | pK18msB-MCS1 containing genomic regions that naturally flank Saro_1814 | This work | |
| pK18msB/ΔSaro1817 | pK18msB-MCS1 containing genomic regions that naturally flank Saro_1817 | This work | |
| pK18msB/ΔSaro0236 | pK18msB-MCS1 containing genomic regions that naturally flank Saro_0236 | This work | |
| pK18msB/ΔSaro1168 | pK18msB-MCS1 containing genomic regions that naturally flank Saro_1168 | This work | |
| pK18msB/ΔSaro0236::StaxiW | pK18msB/ΔSaro0236 with the gene for CrtW from S. taxi ATCC 55669 between the Saro_0236 flanking regions | This work | |
| pK18msB/ΔSaro0236::SastaW | pK18msB/ΔSaro0236 with the gene for CrtW from S. astaxanthinifaciens between the Saro_0236 flanking regions | This work | |