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. 2023 Nov 22;97(12):e01183-23. doi: 10.1128/jvi.01183-23

Fig 7.

Fig 7

JINR1 and RBM10 promote flavivirus replication in human astrocytoma cells. (A) JINR1 promotes JEV replication in T98G cells. Cells were transfected with either ASO-NS, ASO-JINR1-1, or ASO-JINR1-2, and viral replication was determined by quantifying the intracellular levels of JEV RNA at 48 hpi. (B) JINR1 promotes DENV replication in T98G cells. Cells were transfected with either ASO-NS, ASO-JINR1-1, or ASO-JINR1-2, and viral replication was determined by quantifying the intracellular levels of DENV RNA at 48 hpi. (C) JINR1 promotes WNV replication in T98G cells. Cells were transfected with either ASO-NS, ASO-JINR1-1, or ASO-JINR1-2, and viral replication was determined by quantifying the intracellular levels of WNV RNA at 48 hpi. (D) JINR1 silencing reduces JEV titer. Quantification of viral titer upon JINR1 depletion during JEV infection in T98G cells is shown in Fig. S21A. (E) JINR1 silencing reduces DENV titer. Quantification of viral titer upon JINR1 depletion during DENV infection in T98G cells is shown in Fig. S21B. (F) JINR1 silencing reduces WNV titer. Quantification of viral titer upon JINR1 depletion during WNV infection in T98G cells is shown in Fig. S21C. (G) RBM10 promotes JEV replication in T98G cells. Cells were transfected with either si-NS or si-RBM10, and viral replication was determined by quantifying the intracellular levels of JEV RNA at 36 hpi. (H) RBM10 promotes DENV replication. T98G cells were transfected with si-NS or si-RBM10, and viral replication was determined by quantifying the intracellular levels of DENV RNA at 36 hpi. (I) RBM10 promotes WNV replication. T98G cells were transfected with either si-NS or si-RBM10, and viral replication was determined by quantifying the intracellular levels of WNV RNA at 36 hpi. (J) RBM10 silencing reduces JEV titer. Quantification of viral titer upon RBM10 depletion during JEV infection in T98G cells is shown in Fig. S22A. (K) RBM10 silencing reduces DENV titer. Quantification of viral titer upon RBM10 depletion during DENV infection in T98G cells is shown in Fig. S22B. (L) RBM10 silencing reduces WNV titer. Quantification of viral titer upon RBM10 depletion during WNV infection in T98G cells is shown in Fig. S22C. Error bars represent the mean ± SEM from three independent experiments. Statistical comparison was made using the Student’s t-test. (A–C and G–I) RNA samples were analyzed by qRT-PCR. (A and D) *Significant change compared to JEV-ASO-NS. (B and E) *Significant change compared to DENV-ASO-NS. (C and F) *Significant change compared to WNV-ASO-NS. (G and J) *Significant change compared to JEV-si-NS. (H and K) *Significant change compared to DENV-si-NS. (I and L) *Significant change compared to WNV-si-NS.