Figure 1.

Accumulation of WRKY23 and bHLH041 during callus induction. A) Relative WRKY23 and bHLH041 transcript levels in response to CIM. Seven-day-old wild type (WT) seedlings incubated in liquid CIM for the indicated times were used for RNA isolation and RT-qPCR analysis. Data are shown as means ± Sd (n = 3 biological replicates). *P < 0.05, **P < 0.01 by t-test. B, C) Accumulation of WRKY23 and bHLH041 during callus induction. Five-d-old transgenic seedlings carrying a ProWRKY23:gWRKY23-GFP or ProbHLH041:bHLH041-GFP transgene were incubated on CIM for the indicated times. GFP signals from the mature zone of primary roots were overlaid with signals stained with propidium iodide in B), and the total proteins from seedlings were immunoblotted by anti-GFP and anti-ACTIN antibodies for 2 biological replicates in C). Scale bar, 50 µm.