Skip to main content
. 2023 Oct 6;36(1):158–173. doi: 10.1093/plcell/koad255

Figure 2.

Figure 2.

WRKY23 and bHLH041 regulate callus formation and shoot regeneration. A) Callus-forming and shoot-regenerating phenotypes of WT, wrky23-3, wrky23-4, wrky23-5, and wrky23-3 ProWRKY23:gWRKY23-GFP explants and derived calli. B) Callus-forming and shoot-regenerating phenotypes of WT, empty vector (EV), and 35S:WRKY23 explants and derived calli. Four independent 35S:WRKY23 transgenic lines named #1, #2, #3, and #4 were examined. C) Callus-forming and shoot-regenerating phenotypes of WT, bhlh041-D, bhlh041-1, and bhlh041-1 ProbHLH041:bHLH041-GFP explants and derived calli. D) Callus-forming and shoot-regenerating phenotypes of WT, EV, and 35S:bHLH041 explants and derived calli. Three independent 35S:bHLH041 transgenic lines named #1, #2, and #3 were examined. The cotyledon and root explants from 7-d-old seedlings were incubated on CIM for 21 d or for 7 d and subsequently on SIM for 14 d before being examined for callus formation and shoot regeneration, respectively. The callus area of each explant and the number of regenerating shoots from each callus were determined (n = 20 explants). Scale bars, 10 mm. Data are shown as means ± Sd. *P < 0.05, **P < 0.01, and ***P < 0.001 by t-test.