Skip to main content
. 2023 Oct 6;36(1):158–173. doi: 10.1093/plcell/koad255

Figure 3.

Figure 3.

WRKY23 and bHLH041 are activator and repressor of root stem cell genes, respectively. A) Transcript abundance of root stem cell genes in root explants with forming callus from WT, wrky23-3, and 35S:WRKY23 (upper panel) and WT, bhlh041-1, and bhlh041-D (bottom panel). RT-qPCR analysis was performed with root explants incubated on CIM for 7 d (n = 3 biological replicates). B) Accumulation of WOX5 and PLT1 in the callus-forming roots of WT, wrky23-3, and bhlh041-D. Five-d-old transgenic seedlings carrying a ProWOX5:GFP-ER or ProPLT1:PLT1-YFP transgene were incubated on CIM for 4 d, and GFP fluorescent signals were visualized and quantified at the mature zone of primary roots (n = 10 seedlings). The GFP signals were overlaid with the cells stained with propidium iodide. Scale bar, 50 µm. Data are shown as means ± Sd. *P < 0.05, **P < 0.01, and ***P < 0.001 by t-test.