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. 2023 Sep 21;36(1):65–84. doi: 10.1093/plcell/koad246

Figure 2.

Figure 2.

HvMADS8 induces ovule initiation and establishes FM determinacy. A) Semi-thin sections of W9.5 GP and Hvmads8 pistils at 15 °C (horizontal sections) or 28 °C (left, horizontal sections; right, longitudinal sections). ca, carpel; ov, ovule. Scale bars, 100 μm. B) In situ hybridization showing expression of HvMADS13 (ovule marker gene) in GP and Hvmads8 florets at 15 and 28 °C. Asterisks indicate extra carpels in Hvmads8 lines at 28 °C. Scale bars, 100 μm. C) Relative expression of HvMADS13 at early stages of inflorescence development at 15 and 28 °C. Values are means ± Sd; n = 3 biological replicates. Asterisks indicate significant differences (2-way ANOVA test; *P < 0.05, **P < 0.01). D) In situ hybridization showing expression of HvOSH1 (meristematic cell marker gene) in GP and Hvmads8 inflorescences and florets at 15 and 28 °C. le, lemma; st, stamen. Asterisks indicate extra carpels in Hvmads8 lines at 28 °C. Scale bars, 100 μm. E) Relative expression of HvOSH1 at different stages of inflorescence and pistil development at 15 and 28 °C. Values are means ± Sd; n = 3 biological replicates. Asterisks indicate significant differences (2-way ANOVA test; *P < 0.05, **P < 0.01).