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. 2023 Sep 21;36(1):65–84. doi: 10.1093/plcell/koad246

Figure 3.

Figure 3.

Temporal and spatial expression of HvMADS8 at different temperatures. A) Relative expression of HvMADS8 in different GP plant tissues and at different stages of development at 28 °C. Values are means ± Sd; n = 3 biological replicates. B) Relative expression of HvMADS8 at early stages of inflorescence development at 15 and 28 °C. Expression of HB (temperature-responsive gene) was used as positive control. Values are means ± Sd; n = 3 biological replicates. Asterisks indicate significant differences (2-way ANOVA test; **P < 0.01). C) In situ hybridization showing expression of HvMADS8 at stages W5.0 to 6.5 at 28 °C in longitudinal sections of GP florets. The sense probe served as a negative control. ca, carpel; le, lemma; ov, ovule; st, stamen. Scale bars, 100 μm. D) Accumulation of HvMADS8 in spikes and florets from W3.0 to 5.5 in proHvMADS8:HvMADS8-eGFP transgenic lines at 15 °C. cp, carpel primordia; fm, floral meristem; pa, palea; pp, pistil primordia. Scale bars, 100 μm. E) Accumulation of HvMADS8 in W3.0 and W4.0 proHvMADS8:HvMADS8-eGFP transgenic spikes grown at 15 and 28 °C. im, inflorescence meristem. Scale bars, 100 μm. F) Immunoblot analysis of HvMADS8-eGFP abundance in W5.5 to 6.5 spikes from 2 independent proHvMADS8:HvMADS8-eGFP lines grown at 15 and 28 °C. Tubulin served as loading control. All experiments were independently performed at least 3 times with similar results.