Figure 4.

HvMADS8 coordinates the thermal transcriptome programming of floral development. A) Correlation analyses of transcript levels at control and high temperatures at 3 stages of Hvmads8 and GP carpel development (W4.0, W4.5, and W8.0). B) Overlap of 13,216 DEGs responding to genotype and temperature. The blue-shaded region contains genes coregulated by genotype and temperature. C) Volcano plots showing differential gene expression in GP relative to Hvmads8 at 15 and 28 °C at the W4.0, W4.5, and W8.0 stages of inflorescence and carpel development. D) GO term enrichment analysis of DEGs between WT and Hvmads8 at 15 and 28 °C. CK, response to cytokinin; JA, regulation of jasmonic acid–mediated signaling; MS, response to monosaccharides; PST, phosphorelay signal transduction; EPM, export across the plasma membrane; AA, amino acid transport. E) Heatmap representation of expression levels for DEGs involved in carpel and ovule development, or FM regulation; plant hormone regulation; or that encode MADS-box proteins.