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Induction of Omp21 expression in cells grown in mineral medium at high aeration in the fermentor under controlled pH, malic acid, and temperature conditions. Top: total cell protein (μ = 0.53 h−1) and relative oxygen concentration (100% denotes 8.5 mg of O2/liter). Bottom: outer membrane protein patterns in SDS-PAGE from samples taken at different times of growth. Omp21 appears after 10 h at a relative O2 concentration of about 20% and is present in all samples beyond 24 h.
