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SDS-PAGE of Omp21 preparations from mutant strain JL0. Left panel: marker proteins (M) and C8En-extracted outer membrane proteins (OM). Middle panel: Omp21 purified by ionic exchange (Q) and hydroxyapatite chromatography (H). This preparation contains a particularly low level of LPS and almost no conformers at 23 and 24 kDa. Right panel: Omp21 purified by gel filtration (G) and hydroxyapatite chromatography (H). Note the additional conformer at 24 kDa. The protein was silver stained.
