TABLE 3.
Plasmids used in this study
| Plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| pBAD18 | araC oripBR322 Ampr | 19 |
| pDFB19 | plac cheY oripUC9 Ampr | D. F. Blair and H. C. Berg |
| pGD2 | ′flhC motAB cheA′ oripBR322 Tetr | 18 |
| pRL22 | ptrp cheYZ oripBR322 Ampr | 30 |
| pRL22ΔZ | ptrp cheY oripBR322 Ampr | 39 |
| pRBB28 | ptrp cheA oripBR322 Ampr | 11 |
| pRBB30 | ptrp cheAW oripBR322 Ampr | 11 |
| pRBB38 | ptrp cheY oripBR322 Ampr | 3 |
| pRBB48 | plac cheY oripBR322 Ampr | This work |
| pUC19 | oripUC19 Ampr | 50 |
| pWA24 | ′flhC motAB cheA′-Zeor-′cheZ flhB′ oripUC19 Ampr | This work |
| pWA27 | araC paraBAD cheA oripBR322 Ampr | This work |
| pWA28 | araC paraBAD cheAW oripBR322 Ampr | This work |
| pZEro-1 | oripUC Zeor | Invitrogen |