TABLE 4.
Comparison of flagellar rotational bias of isogenic Δ(cheA-cheZ)::Zeor and Δ(cheA-cheY)::XhoI(Tn5) strains expressing various chemotaxis proteins
| Chemotaxis genes expresseda (plasmids and bacteriophages) | Mean flagellar rotational bias ± SEM (strain) in:
|
|
|---|---|---|
| Δ(cheA-cheZ)::Zeor background | Δ(cheA-cheY)::XhoI(Tn5) background | |
| None | 1.00 ± 0.00 (RBB1050) | 1.00 ± 0.00 (RBB1051) |
| Y+ (λDFB19) | 1.00 ± 0.00 (RBB1052) | 1.00 ± 0.00 (RBB1053) |
| T+Y+ (λDFB19) | 0.98 ± 0.02 (RBB1175) | 0.99 ± 0.00 (RBB1176) |
| A+Y+ (pWA27, λDFB19) | 0.51 ± 0.09 (RBB1122) | 0.88 ± 0.06 (RBB1123) |
| W+A+Y+ (pWA28, λDFB19) | 0.20 ± 0.06 (RBB1124) | 0.93 ± 0.04 (RBB1125) |
| T+A+Y+ (pWA27, λDFB19) | 0.72 ± 0.07 (RBB1183) | 0.96 ± 0.03 (RBB1185) |
| T+W+A+Y+ (pWA28, λDFB19) | 0.00 ± 0.00 (RBB1184) | 0.10 ± 0.05 (RBB1186) |
| Y++ (pRBB38) | 0.96 ± 0.03 (RBB1068) | 1.00 ± 0.00 (RBB1069) |
| Y+++ (pRL22ΔZ) | 0.22 ± 0.07 (RBB1167) | 1.00 ± 0.00 (RBB1168) |
| Y++++ (pDFB19) | 0.01 ± 0.00 (RBB1066) | 0.30 ± 0.06 (RBB1067) |
a
T+ cells express Tsr and Trg in either the RBB1041 or the RBB1174 strain background. Strains without T are built up in the RBB1050 and RBB1051 strain backgrounds. +, approximately wild-type amounts of protein; ++, +++, and ++++, increasing amounts of protein.