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. 1998 Aug;180(15):3765–3770. doi: 10.1128/jb.180.15.3765-3770.1998

FIG. 5.

FIG. 5

In vitro runoff transcription of PW and PX. PCR products containing either PW or PX were used as templates for runoff transcription reactions with core RNAP either alone (−) or after supplementation with either ςX (X) or ςW (W). Note that the core RNAP preparation has a low level of activity in the absence of added ς, suggesting that this preparation has some contaminating ςX as noted previously (15).