TABLE 2.
Temperature dependence of growth for P. aeruginosa cells containing pFF1 plasmid mutantsa
| pFF1 construct | Growth at:
|
|||
|---|---|---|---|---|
| 23°C | 30°C | 37°C | 42°C | |
| Wild type | + | + | + | + |
| Mutant 1 | + | + | + | − |
| Mutant 2 | + | − | − | − |
| Mutant 3 | + | − | − | − |
| Mutant 4 | + | − | − | − |
a
P. aeruginosa PAO1161S cells containing the pFF1 plasmids were grown at 23°C in the presence of carbenicillin to stationary phase, diluted, and plated (about 300 cells/plate) on agar medium containing carbenicillin. The plates were incubated overnight (2 days for incubations at 23°C) at the temperatures indicated and then inspected for visible growth.