TABLE 4.
Temperature dependence of growth for E. coli DH5α cells containing trfA single mutants
| Plasmid, trfA form | Growth at:
|
|||
|---|---|---|---|---|
| 23°C | 30°C | 37°C | 42°C | |
| pFF1, wild type | + | + | + | + |
| pPK34, wild type | + | + | + | + |
| pFF1, 163C,235 | − | − | − | − |
| pPK34, 163C,235 | − | − | − | − |
| pFF1, 163C | − | − | − | − |
| pPK34, 163C | (+) | − | − | − |
| pFF1, 235 | + | − | − | − |
| pPK34, 235 | + | + | − | − |
a
The experiment was performed as described in the footnote to Table 2 except that the agar medium contained ampicillin instead of carbenicillin. Cells containing pPK34 or its mutant derivatives also contained pSV16. (+) indicates poor growth.