TABLE 3.

Effect of exogenously added FAD on the metabolism of L. lactis NZ9800(pNZ2600)^a

Product addedb	Final pH	Amt of product produced (mM)			Amt of NADH oxidase overproduced (U/mg)c
		Lactate	Acetate	Acetoin or diacetyl
Control with FAD	4.94	27.5	4.7	0.2	0.31
Control with nisin	5.58	9.0	5.9	9.9	8.29
FAD	5.65	NDd	6.8	11.7	13.67

^aAll experiments were carried out at an initial pH of 6.0. Formate and butanediol were not detected. 

^bNisin (0.4 U ml⁻¹) was not added to the control with FAD. FAD (2 μg ml⁻¹) was not added to the control with nisin. 

^cOverproduction of the heterologous H₂O-forming NADH oxidase. 

^dND, not detected.