FIG. 2.

Phylogenetic tree of trehalose-6-P synthase from different organisms and regions of putative regulatory importance in the CaTPS1 promoter. (A) Amino acid sequences of the various proteins were aligned with the CLUSTAL V program (22). A maximum parsimony consensus tree (100 bootstrap resamplings) was obtained with the PHYLIP 3.5 package (14). The sequences were retrieved from the SWISSPROT database. Numbers at the intersections indicate the bootstrap value as a percentage. The percentage of identity with respect to the C. albicans amino acid sequence is shown at the right. Figures in brackets were obtained by comparing only the 500 initial amino acids in the case of Arabidopsis thaliana or using only amino acids 290 to 415 from C. albicans in the case of Methanobacterium thermoautotrophicum. (B) Part of the 5′ noncoding region of the CaTPS1 gene. Putative regulatory sequences are underlined.