TABLE 1.
Trehalose accumulation in stationary phase and after heat shock in C. albicans and in S. cerevisiae strains expressing the CaTPS1 gene
| Strain (relevant genotype) | Growth temp (°C) | Trehalose (nmol/mg [wet wt] of yeast)a
|
|
|---|---|---|---|
| Stationary phase | Heat shock | ||
| C. albicans SC5314 (wild type) | 30 | 5 | 50 (17b) |
| 37 | 20 | NT | |
| C. albicans (tps1/tps1) | 30 | 2 | 1 |
| 37 | 2 | NT | |
| S. cerevisiae W303 (wild type) | 30 | 20 | 29 |
| S. cerevisiae WDC-3A (tps1) | 30 | <1 | <1 |
| S. cerevisiae (tps1/CaTPS1) | 30 | 18c/22d | 14 |
C. albicans and S. cerevisiae strains were grown in YPD and minimal medium with 2% galactose as the carbon source (S. cerevisiae tps1 strains do not grow in glucose), respectively. Heat shock was performed in exponentially growing cells by a shift of the culture to 42°C during 1 h. In exponential-phase cells of all strains tested, trehalose production was <1 nmol/mg. All C. albicans strains were prototrophic for uracil, but see footnote b. NT, not tested.
Refers to strain CAI4, which is auxotrophic for uracil.
CaTPS1 expressed in the centromeric plasmid pRS316.
CaTPS1 expressed from the multicopy plasmid YEp352.