Skip to main content
. 2005 Apr;16(4):1593–1605. doi: 10.1091/mbc.E04-11-1035

Figure 9.

Figure 9.

The Atg1–Atg13 complex and Atg1 kinase activity affect Atg11 homo-oligomer disassembly. (A) Atg11 homo-oligomers accumulate in the absence of Atg1 or Atg13. atg11Δ (AHY001), atg1Δ (WHY1), atg13Δ (CWY233), or atg20Δ (D3Y109) cells with plasmids expressing CFP-Atg11ΔCC4 and wild-type Atg11, or atg1Δ cells with a plasmid expressing CFP-Atg11ΔCC4 and the Atg1K54A mutant and wild-type Atg11 were observed with a fluorescence microscope, and 200–250 cells containing fluorescent punctate dots were quantified. Error bars indicate the SD of three independent experiments. (B) The absence of Atg1 or Atg13 does not increase the stability of the PAS. Wild-type (SEY6210), atg1Δ (WHY1), atg13Δ (CWY233), or atg20Δ (D3Y109) cells transformed with a plasmid expressing GFP-Atg8 were observed and cells containing punctate dots were quantified. Error bars indicate the SD of three independent experiments.