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Effect of overexpressing SMAP1 protein on the subcellular distribution of adaptor proteins and clathrin. HeLa cells were transfected with the HA-SMAP1 expression plasmid, fixed, and stained with anti-HA antibody together with antibodies specific for AP-2 (A), CHC (B), AP-1 (C), or AP-3 (D). Bar, 20 μm.
