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Ca2+ dependence of the ATPase activity of wild-type CD39 and N-glycosylation site mutants. Crude membranes (10 μg of protein) of COS7 cells expressing wild-type or mutant CD39 were incubated with ATP in the presence of different concentrations of free calcium as described in Grinthal and Guidotti (2000). Released phosphate was assayed according to Ames (1966).
